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IBUN
Instituto de Biotecnología
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Viral molecular Biology

Our group is responsible for diagnosing and the biological, serological and molecular characterisation of phytopathogenous viruses in IBUN. The group has carried out research into citric fruit for detecting and characterising the citrus tristeza virus (CTV). The group has recently adapted serological and/or molecular detection techniques for PVYY, PVX, PVY, PVS and PLRV viruses in potato crops and has developed YMV and YMMV virus detection for yam crops (Dioscorea spp), supported by the agricultural biotechnology programme. We are also able to adapt serological and/or molecular techniques for detecting other pathogens such as viroids and fungi, these being important for ornamental plant crops and/or other crops having nutritional interest. The group is currently providing extension services concerning training in specific diagnostic techniques such as serological, immunoprinting and ELISA techniques, and those derived from amplifying genes by RT-PCR and IC-RT-PCR.

We diagnose and characterise viruses and other pathogens infecting important commercial and ornamental crops for Colombia. Our research results have led to knowing potentially useful strains or genes for being used in control mechanisms.

  • Consolidating research lines concerning the molecular biology of the citric tristeza virus (CTV) and producing viral infection-resistant transgenic potato plants, supported by Colciencias and international cooperation from the University of Florida and the Scottish Crop Research Institute (SCRI).
  • Fostering new human resources at both undergraduate and postgraduate level.
  • Being one of the supports for the successful initiation of the Faculty of Medicine’s MSc Biochemistry and Physiology programmes and (by directing postgraduate theses) contributing our research ability towards developing Microbiology and Phytopathology programmes.

GUZMÁN, M., N. BOONHAM, LÓPEZ, R., ASENCIO C. Development of a real-time RT-PCR assay for the detection of potato yellow vein virus (PYVV). Journal of Virological Methods. Elsevier.com: v.136, n.1, p.24 - 29, 2006.
GUZMÁN, M., RUIZ E. ARCINIÉGAS N., RHA COUTTS. Ocurrence and variability of potato yellow vein virus in the three departments of Colombia. Journal of Phytopathology. Blackwell Publishing: v.154, n.1, p.748 - 750, 2006.
GUZMÁN, M., R. COUTTS, MULLER, G., ELIASCO, E., LIVIERATOS, C. Sequences of defective RNAs associated with potato yellow vein virus. Archives of Virology. Springer Wien: v.151, n.1, p.201 - 204, 2006.
GUZMÁN, M., OFFEII, S.K., R. COUTTS, SALAZAR L., MULLER, G., ARCINIÉGAS N., Molecular variation of potato yellow mosaic virus isolates. Brief Report. Archives of Virology: v.149, p.821 - 827, 2004.
GUZMÁN, M., PEÑARANDA, ACOSTA O., OLIVEROS O., TORRES Y., CHAPARRO, O. Obtención de un anticuerpo contra la proteína p65 del virus de la tristeza de los cítricos y resultados preliminares de la expresión in vitro. Revista Colombiana de Biotecnología. Unibiblios: v.2, n.1, p.32 - 39, 2003.
GUZMÁN, M., CARO, M. , GARCÍA Y . Técnica de Inmunoimpresión en membrana de nitrocelulosa, una técnica rápida para estimar la incidencia de los virus PVX, PVY, PVS, PLRV que infectan a la papa (Solanum spp). Revista Colombiana de Biotecnología, v.4, n.2, p.15 - 20, 2002.

Universidad Nacional de Colombia
Carrera 45 No 26-85 - Edificio Uriel Gutiérrez
Bogotá D.C. - Colombia